Primordial follicles

1. Caloric restriction decreases primordial follicles in mice (-60%).

   “fewer primordial follicles (60% reduction; P=0.001), transitional follicles (P=0.0006) and total follicles (P=0.006) were observed in offspring from CR mothers.”

   Animalsgestational calorie restriction from Day 10 until delivery50% of control dietovaryDay 10 of gestation until deliveryfemales

   Calorie restriction during gestation affects ovarian reserve in offspring in the mouse

   Reproduction, fertility, and development·1 Dec 2020

2. Caloric restriction decreases primordial follicles in rats.

   “The number of the primordial follicle was lower in FR1 and FR2 females vs. controls at both week 4 and at week 8.”

   Animalsmaternal food restriction throughout gestationovaryfemales

   Food restriction during pregnancy and female offspring fertility: adverse effects of reprogrammed reproductive lifespan

   Journal of ovarian research·28 Dec 2017

3. Caloric restriction increases primordial follicles in rats.

   “both the 25 and 45% CR rats had a significantly higher percentage of primordial follicles and a larger number of healthy follicles than the NC rats”

   Animalsovary2 monthsfemalesn = 48

   The effects of caloric restriction and a high-fat diet on ovarian lifespan and the expression of SIRT1 and SIRT6 proteins in rats

   Aging clinical and experimental research·14 Apr 2011

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1. 17-alpha-estradiol has no effect on primordial follicles in mice.

   “however had no effect in wild type mice.”

   Animalsin wild type micein the dietovaryfour monthsfemales

   17α-Estradiol promotes ovarian aging in growth hormone receptor knockout mice, but not wild-type littermates

   Experimental gerontology·4 Nov 2019

2. 17-alpha-estradiol decreases primordial follicles in mice (p < 0.05).

   “17α-E2 treatment decreased the number of primordial follicles in GHRKO mice (p < 0.05), however had no effect in wild type mice.”

   Animalsin GHRKO micein the dietovaryfour monthsfemales

   17α-Estradiol promotes ovarian aging in growth hormone receptor knockout mice, but not wild-type littermates

   Experimental gerontology·4 Nov 2019

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1. Dactolisib increases primordial follicles in mice.

   “In vivo, ovaries slow-frozen with BEZ235 retained a higher percentage of primordial follicles”

   Animalsthree weeks after autotransplantation under kidney capsulethree weeks

   BEZ235-Mediated PI3K/mTOR dual inhibition improves ovarian follicle survival in a preclinical model

   Reproductive biology and endocrinology : RB&E·19 Jun 2025

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1. SRT1720 increases primordial follicles in mice.

   “SRT mice had significantly greater number and percentage of primordial follicles, but lower number and percentage of corpora lutea and atretic follicles than the CHF mice”

   Animalsdiet-induced obesityovary6 weeksfemales

   SIRT1 activator (SRT1720) improves the follicle reserve and prolongs the ovarian lifespan of diet-induced obesity in female mice via activating SIRT1 and suppressing mTOR signaling

   Journal of ovarian research·21 Oct 2014

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1. Genistein increases primordial follicles in rats.

   “Genistein-treated rats obtained a higher percentage of primordial follicles by 4 months of age”

   Animalsovary160 mg/kg d4 weeksfemales

   Effects of genistein on ovarian follicular development and ovarian life span in rats

   Fitoterapia·1 Jul 2010

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1. Tea polyphenols increase primordial follicles in rats.

   “The percentage of primordial follicles increased, while that of developing follicles decreased in the ovaries of 4- and 8-day-old rats following peritoneal injection with tea polyphenols compared with controls.”

   Animalsin 4- and 8-day-old ratsovary

   Effects of tea polyphenols on ovarian development in rats

   Journal of endocrinological investigation·1 Dec 2008

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1. SIRT1 increases primordial follicles in mice.

   “mean number and percentage of primordial follicles in both the homozygous and heterozygous SIRT1-overexpressing mice were higher than wild-type mice at the same age”

   Animalsoocyte-specific overexpressionovaryfemales

   SIRT1 knock-in mice preserve ovarian reserve resembling caloric restriction

   Gene·17 Oct 2018

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1. BMF decreases primordial follicles in mice.

   “Bmf(-/-) mice had significantly more primordial follicles than wild-type (WT) control animals at Postnatal Days 100, 200, 300, and 400”

   Animalsshown by knockoutovaryfemales

   Loss of the proapoptotic BH3-only protein BCL-2 modifying factor prolongs the fertile life span in female mice

   Biology of reproduction·10 Apr 2014

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