Association of sirtuin 1 rs10997868 and rs730821 polymorphisms with sirtuin 1 and hydroxy-2'-deoxyguanosine levels in healthy smokers: A case-control study

ObjectivesThis case-control study explored the association of sirtuin 1 polymorphisms (rs10997868 and rs730821) with metabolic parameters, smoking duration, sirtuin 1 levels, and the oxidative stress biomarker 8-hydroxy-2'-deoxyguanosine in healthy smokers in Saudi Arabia.MethodsGenomic DNA was isolated from peripheral blood samples, and rs10997868 and rs730821 polymorphism genotyping was performed for 43 healthy smokers and 33 healthy controls using real-time polymerase chain reaction. Plasma sirtuin 1 and 8-hydroxy-2'-deoxyguanosine levels were measured using enzyme-linked immunosorbent assay.ResultsSirtuin 1 levels were significantly higher in healthy smokers than in controls, while 8-hydroxy-2'-deoxyguanosine levels were not significantly lower in healthy smokers. There was no significant association of sirtuin 1 protein expression and 8-hydroxy-2'-deoxyguanosine levels with rs10997868 and rs730821. Our findings showed a significant association between smoking duration and rs10997868 single nucleotide polymorphism, which was highest in those with the C/C genotype and lowest in those with the A/C genotype (p = 0.035). In addition, the serum cholesterol level was highest in the C/C group and lowest in the A/C group (p = 0.029). None of the studied factors (smoking duration, serum cholesterol, fasting blood sugar, or serum triglycerides) was significantly associated with sirtuin 1 rs730821.ConclusionsOur study showed that sirtuin 1 upregulation and polymorphisms are associated with smoking duration and lipid levels in healthy smokers.