p70S6K phosphorylation

1. Resistance training increases p70S6K phosphorylation in rats.

   “p70S6K phosphorylation exhibited a significant main effect of RE and MR, with higher values observed across both conditions.”

   Animalsskeletal musclemales

   Eight-week dietary methionine restriction does not impair resistance exercise-induced mTORC1 signalling activation in rats

   Biochemistry and biophysics reports·Jan 31, 2026

2. Resistance training increases p70S6K phosphorylation in rats (healthy rats without tumor).

   “in healthy rats, it promoted activated mTORC1, as demonstrated by p70S6K's increased phosphorylation and myofiber's enlarged cross-sectional area”

   Animalshealthy rats without tumorladder climbingskeletal muscle

   Resistance Training's Ability to Prevent Cancer-induced Muscle Atrophy Extends Anabolic Stimulus

   Medicine and science in sports and exercise·Mar 16, 2021

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1. Voluntary wheel running increases p70S6K phosphorylation in wild-type mice, depending on mTOR activation.

   “The levels of p70S6K and 4EBP1 phosphorylation were declined in Akt3-KO mice and elevated in running WT mice”

   Animalsin wild-type mice, depending on mTOR activation7 days

   Akt3-mTOR regulates hippocampal neurogenesis in adult mouse

   Journal of neurochemistry·Jun 3, 2021

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1. Everolimus inhibits p70S6K phosphorylation in vitro and in vivo.

   “phosphorylation of p70S6K was almost completely blocked both in vitro and in vivo”

   Animalsin vitro and in vivo

   The Altered Metabolic Molecular Signatures Contribute to the RAD001 Resistance in Gastric Neuroendocrine Tumor

   Frontiers in oncology·Apr 21, 2020

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1. Branched-chain amino acids increase p70S6K phosphorylation in rats (combined with endurance training in LPD-fed CKD rats) (restored to sham-group level).

   “BCAA supplementation of the LPD-fed, exercise-trained CKD rats restored the phosphorylation of p70s6 kinase to the same level observed in the sham group”

   Animalscombined with endurance training in LPD-fed CKD ratsBCAA-fortified low-protein diet (BD)soleus muscle

   Effect of endurance training and branched-chain amino acids on the signaling for muscle protein synthesis in CKD model rats fed a low-protein diet

   American journal of physiology. Renal physiology·Jul 12, 2017

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1. Endurance training increases p70S6K phosphorylation in LPD-fed CKD rats compared with sham (slight).

   “The treadmill exercise slightly increased the phosphorylation of p70s6 kinase, a marker of mTOR activity, in the soleus muscle of LPD-fed CKD rats compared with the sham group.”

   Animalsin LPD-fed CKD rats compared with shamtreadmill runningsoleus muscle15 m/min, 1 h/day, 5 days/wk7 wk

   Effect of endurance training and branched-chain amino acids on the signaling for muscle protein synthesis in CKD model rats fed a low-protein diet

   American journal of physiology. Renal physiology·Jul 12, 2017

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1. Rapamycin + resveratrol decreases p70S6K phosphorylation in human cells (compared with rapamycin alone).

   “resulted in decreased p70S6K phosphorylation compared to rapamycin incubation alone”

   Cellscompared with rapamycin alonelipoma cells

   Resveratrol Potentiates Growth Inhibitory Effects of Rapamycin in PTEN-deficient Lipoma Cells by Suppressing p70S6 Kinase Activity

   Nutrition and cancer·Feb 17, 2016

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1. Resveratrol decreases p70S6K phosphorylation in human cells (PTEN-deficient lipoma cells).

   “p70S6 kinase (p70S6K) phosphorylation was reduced in PTEN-deficient lipoma cells after resveratrol incubation”

   CellsPTEN-deficient lipoma cellslipoma cells

   Resveratrol Potentiates Growth Inhibitory Effects of Rapamycin in PTEN-deficient Lipoma Cells by Suppressing p70S6 Kinase Activity

   Nutrition and cancer·Feb 17, 2016

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1. Metformin decreases p70S6K phosphorylation in humans (prediabetes; comparison with placebo).

   “Compared to placebo, metformin increased SIRT1 protein expression and reduced p70S6K phosphorylation (a proxy of mTOR activity).”

   Human trialprediabetes; comparison with placeboperipheral blood mononuclear cells1500 mg/day2 monthsn = 38

   Metformin improves putative longevity effectors in peripheral mononuclear cells from subjects with prediabetes. A randomized controlled trial

   Nutrition, metabolism, and cardiovascular diseases : NMCD·Mar 24, 2015

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1. Omega-3 fatty acids increase p70S6K phosphorylation in humans during hyperaminoacidemic-hyperinsulinemic clamp.

   “greater increases in muscle mTOR(Ser2448) (P = 0.08) and p70s6k(Thr389) (P < 0.01) phosphorylation”

   Human trialduring hyperaminoacidemic-hyperinsulinemic clampskeletal muscle8 wk

   Dietary omega-3 fatty acid supplementation increases the rate of muscle protein synthesis in older adults: a randomized controlled trial

   The American journal of clinical nutrition·Dec 15, 2010

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