MTHFR

3 papers6 findings

C, W, M, H: cells, worms or flies, mice, humans. Dark: independent labs found the same change there. Light: one lab or one study. Outline: nothing yet.

Labs are independent when their papers share no last author and no institution.

Against: findings that point the other way from most findings on the same outcome.

On aging: the drug’s trials ARN judged to be about aging, of every trial that tests it for any use.

No results posted: trials that ended more than a year ago with no results on ClinicalTrials.gov and no paper in ARN’s record.

Company ties: findings with an author at a company, or from a paper whose competing-interests statement declares ties to one.

A gene’s or a process’s line counts the gene with the processes named after it, as NLRP3 with the NLRP3 inflammasome, and the trials of drugs aimed at it.

3 independent labs in 1 country

Humans1
Animals2

Effects

Cognitive function

no changein mice1

1 study

MTHFR has no effect on cognitive function in mice.
“Female Mthfr +/‐ mice showed no significant differences from WT counterparts in these behavior tests.”
Animalsheterozygous knockoutfemalesn = 36
Sex‐Specific Differences in Cognition of Mthfr Deficient Mice
AAIC 2025 abstract25 Dec 2025

Novel object recognition exploratory ratio

upin mice1

1 study

MTHFR increases novel object recognition exploratory ratio in mice (60.591% vs 30.884% (p = 0.0227)).
“For novel object recognition test, Mthfr +/‐ males averaged an exploratory ratio of 60.591% while WT males averaged 30.884% ( p = 0.0227).”
Animalsheterozygous knockoutmalesn = 36
Sex‐Specific Differences in Cognition of Mthfr Deficient Mice
AAIC 2025 abstract25 Dec 2025

Time in open field center

upin mice1

1 study

MTHFR increases time in open field center in mice (121.889 s vs 77.444 s (p = 0.0256)).
“Mthfr +/‐ male mice averaged 121.889 seconds in the open field's center, while male WT mice averaged 77.444 seconds ( p = 0.0256).”
Animalsheterozygous knockoutmalesn = 36
Sex‐Specific Differences in Cognition of Mthfr Deficient Mice
AAIC 2025 abstract25 Dec 2025

Homocysteine

upin mice1

1 study

MTHFR increases homocysteine in mice.
“Reduced enzymatic activity of MTHFR*677C>T prevented homocysteine conversion, elevating blood homocysteine levels.”
Animalshomozygous for humanized Mthfr*677C>T allele, humanized Abeta, APOEe4, and Trem2*R47Hblood
A Gene X Environment Study of a Novel Preclinical Mouse Model: The Humanized Mthfr*677C>T Allele and Inorganic Arsenic in Late‐Onset Alzheimer's Disease Progression
AAIC 2025 abstract24 Dec 2025

Associations

Alzheimer's disease

upin mice1

1 study

MTHFR is associated with Alzheimer's disease in mice.
“Aged LOAD2. Mthfr*677C>T mice showed transcriptional and proteomic signatures in the brain that aligned significantly with those observed in human AD.”
AnimalsLOAD2. Mthfr*677C>T micebrain
A Gene X Environment Study of a Novel Preclinical Mouse Model: The Humanized Mthfr*677C>T Allele and Inorganic Arsenic in Late‐Onset Alzheimer's Disease Progression
AAIC 2025 abstract24 Dec 2025