Reproducibility
Biomarker1 paper1 finding
- Humans1
Upstream
1DNA input
downin humans1
1 study
DNA input
downin humans1
DNA input decreases reproducibility in humans.
“The amplicon concentrations, read depths, and reproducibility between duplicate determinations were significantly lower with 1 ng DNA input compared to inputs of 5-50 ng DNA.”
Humanscompared to inputs of 5-50 ng DNAblood1 ng
Comparison of Four Bisulfite Conversion Kits and Their Effects on DNA Methylation Detection and Age Prediction · Genes · 11 Sep 2026
Latest
46Disease mutations shift epigenetic age in organoids
In a preprint using iPSC-derived organoids, serial passaging re-established aging trajectories while disease-associated mutations drove additional…In a preprint using iPSC-derived organoids, serial passaging re-established aging trajectories while disease-associated mutations drove additional cell-intrinsic shifts in epigenetic age.
DNA polymerase kappa supports genome repair in cultured neurons
In mouse and human neurons, depleting POLK reduced repair-associated DNA synthesis and induced senescence-like stress, while augmenting POLK lowered…In mouse and human neurons, depleting POLK reduced repair-associated DNA synthesis and induced senescence-like stress, while augmenting POLK lowered DNA damage markers.
Aspirin initiation before 65 links to lower Alzheimer's risk
Across electronic health records and insurance claims databases, starting aspirin before age 65 was consistently associated with lower incident…Across electronic health records and insurance claims databases, starting aspirin before age 65 was consistently associated with lower incident Alzheimer’s disease risk.
Persistent depression links to faster frailty accumulation
Persistent high depressive symptoms predicted faster non-cognitive frailty accumulation at 1.42 × 10-3/year and elevated odds of incident frailty…Persistent high depressive symptoms predicted faster non-cognitive frailty accumulation at 1.42 × 10-3/year and elevated odds of incident frailty across three aging cohorts.
Brain diffusivity modifies plasma pTau217 links to pathology
In 602 discovery and 2,313 validation participants, associations between plasma pTau217 and brain pathology were stronger among people with lower…In 602 discovery and 2,313 validation participants, associations between plasma pTau217 and brain pathology were stronger among people with lower periventricular diffusivity.
Automated tool counts teeth from routine brain MRI
The model counted teeth with a mean absolute error of 1.96 and matched manual associations with cognitive scores in a 233-person cohort.The model counted teeth with a mean absolute error of 1.96 and matched manual associations with cognitive scores in a 233-person cohort.
Col4a2 mutation disrupts cerebral artery function in aged mice
In 12-month-old Col4a2 mutant mice, cerebral pial arteries showed impaired myogenic tone driven by PKA-dependent remodeling of ryanodine receptor…In 12-month-old Col4a2 mutant mice, cerebral pial arteries showed impaired myogenic tone driven by PKA-dependent remodeling of ryanodine receptor clusters.
DNA methylation variability provides a distinct epigenetic marker of human aging heterogeneity
Combining methylation variance with mean methylation levels improved predictions of all-cause mortality across two independent human cohorts.Combining methylation variance with mean methylation levels improved predictions of all-cause mortality across two independent human cohorts.