Comparison of Four Bisulfite Conversion Kits and Their Effects on DNA Methylation Detection and Age Prediction

Figure 1. Boxplot of the 6-plex and 4-plex post-PCR concentrations (ng/µL) of the 50 ng cohort (DNA methylation standards and samples) across the four tested bisulfite conversion kits (Fast, Max, Gold, and…
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Figure 1. Boxplot of the 6-plex and 4-plex post-PCR concentrations (ng/µL) of the 50 ng cohort (DNA methylation standards and samples) across the four tested bisulfite…Boxplot of the 6-plex and 4-plex post-PCR concentrations (ng/µL) of the 50 ng cohort (DNA methylation standards and samples) across the four tested bisulfite conversion kits (Fast, Max, Gold, and Premium).Giuffrida et al. · CC BY

Genes

Abstract

Background/Objectives : This study aimed to compare the performance of four commercial bisulfite conversion kits-the Premium Bisulfite Kit, the SuperMethyl™ Fast Bisulfite Conversion Kit, the SuperMethyl™ Max Bisulfite Conversion Kit, and the EZ DNA Methylation-Gold™ Kit (hereafter: Premium, Fast, Max, and Gold)-to eva

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