Lymphocyte aging in allophenic mice

Allophenic mice are chimeras which are produced from the aggregation of two genotypically distinct embryos. In this study, embryos from the C57BL/6J and A/J strains were used to produce C57BL/6J----A/J allophenic mice. These strains were chosen because of their markedly different lifespans, their different glucose phosphate isomerase (GPI) isozymes, and their different H-2 haplotypes [C57BL/6J: long-lived, Gpi-1b, H-2b; A/J: short-lived, Gpi-1a, H-2a]. The mice were bled at two month intervals and the composition of their peripheral blood lymphocytes determined at each point from analysis of GPI isozymes and H-2 antigens. It was found that the proportion of long-lived (C57BL/6J) lymphocytes tended to increase with age. Moreover, the total lifespan of the allophenic mice was directly related to the percentage of long-lived lymphocytes in their peripheral blood.