Y-27632

1. Y-27632 increases proliferation in humans.

   “addition of the ROCK inhibitor Y-27632 enhances proliferation and culture longevity”

   Reviewin 3T3-J2 fibroblast feeder cell co-culture systems

   WS6 enables scalable ex vivo expansion and gene editing of human basal epithelial cells

   bioRxiv (Cold Spring Harbor Laboratory)·15 Sep 2025Preprint

1. Y-27632 induces oncogene-induced senescence in human cells.

   “treatment with RhoA/ROCK inhibitors such as Y-27632 destabilized not only cytoskeleton but also the nuclear envelope to induce OIS even under hypoxic condition.”

   Cellsunder hypoxic conditionIMR90 human fibroblasts

   Hypoxic potentiation of cytoskeleton prevents oncogene-induced senescence

   Research Square·30 Nov 2022Preprint

1. Y-27632 decreases keratinocyte differentiation in human cells.

   “Y-27632, significantly suppresses keratinocyte differentiation and extends life span in serum-containing medium”

   Cellsskin

   Radiation induces diffusible feeder cell factor(s) that cooperate with ROCK inhibitor to conditionally reprogram and immortalize epithelial cells

   The American journal of pathology·3 Oct 2013

1. Y-27632 decreases TR90 in mice.

   “aging-associated prolongation in TR90 was blunted by the Rho kinase inhibitor Y-27632”

   Animalsin aged cardiomyocytes

   Metallothionein prolongs survival and antagonizes senescence-associated cardiomyocyte diastolic dysfunction: role of oxidative stress

   FASEB journal : official publication of the Federation of American Societies for Experimental Biology·3 Apr 2006

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