Passage

1. Passage decreases cell cycling fraction in human cells.

   “we show increased p16 expression with subsequent passage as well as a reduction in cycling cells and open chromatin marks.”

   Cellsbone marrow

   Mass Cytometry as a Tool for Investigating Senescence in Multiple Model Systems

   Cells·Aug 11, 2023

1. Passage increases CDKN2A in human cells.

   “In bone marrow mesenchymal stromal cells (BMSCs), we show increased p16 expression with subsequent passage as well as a reduction in cycling cells and open chromatin marks.”

   Cellswith subsequent passagebone marrow

   Mass Cytometry as a Tool for Investigating Senescence in Multiple Model Systems

   Cells·Aug 11, 2023

More on CDKN2A

1. Passage increases extracellular lactate in human cells.

   “Metabolic profiling by NMR showed increased levels of extracellular lactate, phosphocholine and glycine in cells at later passages.”

   Cellslate passage cells (passage 17 vs passage 4)fibroblastsfrom passage 4 to passage 17

   The Long-Term Culture of Human Fibroblasts Reveals a Spectroscopic Signature of Senescence

   International journal of molecular sciences·May 23, 2022

More on Extracellular lactate

1. Passage decreases triglyceride in human cells.

   “Late passage cells were characterized by a decrease in the length of fatty acid chains, triglycerides and cholesterol and an increase in lipid unsaturation.”

   Cellslate passage cells (passage 17 vs passage 4)fibroblastsfrom passage 4 to passage 17

   The Long-Term Culture of Human Fibroblasts Reveals a Spectroscopic Signature of Senescence

   International journal of molecular sciences·May 23, 2022

More on Triglyceride