Loadless culture

1. Loadless culture decreases collagen I in human cells.

   “Collagen I gradually decreased and was barely detectable by day 3”

   Cellstendon

   Loadless Tendon Explant Model Exhibits Spontaneous Degradative Phenotypes: An In Vitro Study of Human Tendons

   Journal of clinical medicine research·26 Nov 2025

More on Collagen I

1. Loadless culture decreases collagen fiber density in human cells.

   “collagen fibers also gradually became loose and fractured, contributing to a significant decrease of collage density from day 9.”

   Cellstendon

   Loadless Tendon Explant Model Exhibits Spontaneous Degradative Phenotypes: An In Vitro Study of Human Tendons

   Journal of clinical medicine research·26 Nov 2025

1. Loadless culture increases CASPASE-3 in human cells.

   “senescence and apoptosis markers P21 and Caspase 3 increased over time, and there were statistically significant differences starting from day 6, respectively.”

   Cellstendon

   Loadless Tendon Explant Model Exhibits Spontaneous Degradative Phenotypes: An In Vitro Study of Human Tendons

   Journal of clinical medicine research·26 Nov 2025

More on CASPASE-3

1. Loadless culture increases P21 in human cells.

   “senescence and apoptosis markers P21 and Caspase 3 increased over time, and there were statistically significant differences starting from day 6, respectively.”

   Cellstendon

   Loadless Tendon Explant Model Exhibits Spontaneous Degradative Phenotypes: An In Vitro Study of Human Tendons

   Journal of clinical medicine research·26 Nov 2025

More on P21