Electroacupuncture attenuates anesthesia/surgery-induced cognitive impairment in aged rats by inhibiting IL-17A mediated hippocampal synaptic dysfunction via the ERK/CREB signaling pathway

Electroacupuncture (EA) has been identified as an effective intervention for mitigating postoperative cognitive dysfunction in the elderly; however, its underlying mechanisms remain inadequately understood. This study aimed to elucidate the neuroprotective effects of EA on anesthesia/surgery-induced cognitive impairment in aged rats through a combination of behavioral testing, RNA sequencing analysis, Golgi-Cox staining, dendritic spine analysis, Sholl analysis, immunofluorescence assays, and western blot analysis. The findings demonstrated that EA ameliorates anesthesia/surgery-induced cognitive decline by inhibiting hippocampal synaptic dysfunction, with a notable enrichment in the downregulation of IL-17A signaling and upregulation of ERK signaling. Furthermore, the study confirmed that an exogenous increase in IL-17A expression can induce hippocampal synaptic dysfunction and partially negate the neuroprotective effects of EA by inhibiting the expression of the ERK/CREB signaling pathway. In conclusion, EA attenuates anesthesia/surgery-induced cognitive impairment in aged rats by inhibiting IL-17A-mediated hippocampal synaptic dysfunction via the ERK/CREB signaling pathway.