GSK343

1. GSK343 increases primary chondrocyte proliferation in human cells.

   “Functionally, conditioned media from GSK343-treated MSCs significantly increased primary chondrocyte proliferation, demonstrating preserved therapeutic potency.”

   Cellsconditioned media from GSK343-treated MSCs

   Mechanoepigenetic Targeting of Histone Methyltransferase EZH2 Increases Potential of Scalable Manufacturing in Human Derived Primary Mesenchymal Stromal Cells

   bioRxiv : the preprint server for biology·12 Dec 2025Preprint

1. GSK343 inhibits cellular senescence in human cells.

   “RNA-seq demonstrated that GSK343 maintained transcriptional programs associated with immunomodulation, migration, and trophic signaling, while suppressing hyperproliferative and senescence-associated genes that are characteristic of late-passage MSCs.”

   Cellsin late-passage MSCs

   Mechanoepigenetic Targeting of Histone Methyltransferase EZH2 Increases Potential of Scalable Manufacturing in Human Derived Primary Mesenchymal Stromal Cells

   bioRxiv : the preprint server for biology·12 Dec 2025Preprint

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1. GSK343 improves stem cell maintenance in human cells.

   “Pharmacological inhibition of EZH2 with GSK343 selectively reduced H3K27me3, restored ARID1A-containing SWI/SNF organization, and preserved MSC morphology and expression of canonical stemness markers (CD73, CD90, CD105) even at later passage.”

   Cellsat later passage during expansion

   Mechanoepigenetic Targeting of Histone Methyltransferase EZH2 Increases Potential of Scalable Manufacturing in Human Derived Primary Mesenchymal Stromal Cells

   bioRxiv : the preprint server for biology·12 Dec 2025Preprint

More on Stem cell maintenance

1. GSK343 decreases H3K27me3 in human cells.

   “Pharmacological inhibition of EZH2 with GSK343 selectively reduced H3K27me3, restored ARID1A-containing SWI/SNF organization, and preserved MSC morphology and expression of canonical stemness markers (CD73, CD90, CD105) even at later passage.”

   Cellsin-vitro expansion on standard tissue-culture plastic

   Mechanoepigenetic Targeting of Histone Methyltransferase EZH2 Increases Potential of Scalable Manufacturing in Human Derived Primary Mesenchymal Stromal Cells

   bioRxiv : the preprint server for biology·12 Dec 2025Preprint

More on H3K27me3