Ammonium chloride

1. Ammonium chloride decreases cellular senescence in human cells.

   “in primary human aortic smooth muscle cells (HAoSMCs), NH4Cl treatment reduced phosphate-induced mRNA expression of RUNX2/CBFA1, ALPL, and senescence-associated molecules”

   Cellsphosphate-induced

   NH4Cl Treatment Prevents Tissue Calcification in Klotho Deficiency

   Journal of the American Society of Nephrology : JASN·2 Feb 2015

2. Ammonium chloride decreases cellular senescence in mice.

   “reversed the increase of Runx2/Cbfa1, Alpl, Tgfb1, Pai-1, p21, and Glb1 expression in aorta of kl/kl mice”

   Animalsin klotho-hypomorphic (kl/kl) miceaorta

   NH4Cl Treatment Prevents Tissue Calcification in Klotho Deficiency

   Journal of the American Society of Nephrology : JASN·2 Feb 2015

More on Cellular senescence

1. Ammonium chloride prevents tissue calcification in mice.

   “NH4Cl treatment in drinking water (0.28 M) prevented soft tissue and vascular calcification”

   Animalsin klotho-hypomorphic (kl/kl) micein drinking water0.28 M

   NH4Cl Treatment Prevents Tissue Calcification in Klotho Deficiency

   Journal of the American Society of Nephrology : JASN·2 Feb 2015

1. Ammonium chloride extends lifespan in mice (>12-fold).

   “NH4Cl treatment in drinking water (0.28 M) prevented soft tissue and vascular calcification and increased the life span of kl/kl mice >12-fold in males”

   Animalsin klotho-hypomorphic (kl/kl) micein drinking water0.28 Mmales

   NH4Cl Treatment Prevents Tissue Calcification in Klotho Deficiency

   Journal of the American Society of Nephrology : JASN·2 Feb 2015