The extracellular matrix of senescent stromal cells decreased secretion of SDF-1 and HGF by endothelium
Abstract
The process of mesenchymal stromal cells (MSCs) senescence is accompanied by alterations in their extracellular matrix (ECM), which has the potential to compromise cellular interactions within the perivascular niche. In this study, the effect of the ECM of senescent MSCs obtained in a «stress-induced model» on the paracrine activity of endothelial cells (ECs) in vitro was investigated. Senescent MSCs exhibited reduced production of structural and adhesive ECM proteins (including type I collagens, fibulins, perlecan, et al.) and increased deposition of senescence-associated molecules (PAI-1, GDF-15). When ECs were cultured on decellularized ECM of senescent MSCs, a significant decrease in the secretion of angiogenic and chemoattractant factors (SDF-1, HGF) was observed compared to young ECM. These findings highlight a potential role of ECM-mediated mechanisms in the development of age-related vascular pathologies and may provide a foundation for the design of anti-senescent therapeutic strategies.
