Detection of Cellular Senescence on Murine Muscle Tissue Sections by Senescence-Associated β-Galactosidase Staining

Staining for the presence of senescence-associated beta-galactosidase (SA-β-gal) serves as a crucial indicator for cellular senescence. This staining assay is based on the elevated activity of β-galactosidase in senescent cells, which cleaves the X-Gal substrate to produce an insoluble blue product in acidic conditions. This enables X-Gal to be visualized using microscopy. In this study, we describe the identification of SA-β-gal⁺ cells within murine muscle tissues.