Human ovarian tissue explant cultures (static or fluidic conditions) v2

The Cellular Senescence Network (SenNet) was recently established to map senescent cells in the human body. As part of this initiative, our goal is to profile senescent cells in the human ovary in vivo as well as ex vivo using a microphysiological platform called LATTICE. Human ovarian tissue is collected through the Northwestern University Reproductive Tissue Library. Tissue is collected from women undergoing bilateral salpingo-oophorectomies or hysterectomies with absence of ovarian neoplasia. With this model, we are generating a molecular signature of Doxorubicin-induced cellular senescence in static and fluidic culture which will then be mapped onto human ovaries across an aging series. To do this, human ovarian explants (cortex and medulla) are cultured in Doxorubicin (0.1 μg/ml) for 24 hours followed by up to 11 days of culture.