In vitro maturation
In vitro maturation has been studied in 10 papers since 2009: 1 trial and 1 observational study in people, 3 in animals, 2 in cells, 2 reviews. The newest paper, Apr 17, 2025: “Nicotinamide mononucleotide biosynthesis and the F-actin cytoskeleton regulate spindle assembly and oocyte maturation quality in post-ovulatory aged porcine oocytes”.
10 papers study it4 findingsUpdated
None of its findings points the other way.
- 2 independent labs in 2 countries
- Funders: National Institute of Child Health and Human Development and 1 more
- Human trial1
- Humans1
- Animals3
- Cells2
What did studies of In vitro maturation find?
4Studies of In vitro maturation measured 4 outcomes; the most studied are oocyte maturation (1 study), cardiac output (1) and pulse rate (1).
Oocyte maturation
upin human cells1+35%
1 study
Oocyte maturation
upin human cells1+35%
In vitro maturation increases oocyte maturation in human cells (35%).
“Of the harvested germinal vesicle oocytes, 35% developed with simple culture media into mature metaphase II oocytes.”
Cellsovary tissuefemalesn = 13
Reproductive biomedicine online
Cardiac output
downin mice1
1 study
Cardiac output
downin mice1
In vitro maturation decreases cardiac output in mice (optimized in vitro matured group compared with in vivo matured group) (P = 0.0197).
“pulse rate and cardiac output were slightly, but significantly, reduced in the optimized in vitro matured group compared with the in vivo matured group (P = 0.0119 and P = 0.0197, respectively).”
Animalsoptimized in vitro matured group compared with in vivo matured group
Effect of in vitro maturation of mouse oocytes on the health and lifespan of adult offspring
Human reproduction (Oxford, England)
Pulse rate
downin mice1
1 study
Pulse rate
downin mice1
In vitro maturation decreases pulse rate in mice (optimized in vitro matured group compared with in vivo matured group) (P = 0.0119).
“pulse rate and cardiac output were slightly, but significantly, reduced in the optimized in vitro matured group compared with the in vivo matured group (P = 0.0119 and P = 0.0197, respectively).”
Animalsoptimized in vitro matured group compared with in vivo matured group
Effect of in vitro maturation of mouse oocytes on the health and lifespan of adult offspring
Human reproduction (Oxford, England)
Lifespan
no changein mice1
1 study
Lifespan
no changein mice1
In vitro maturation showed no clear effect on lifespan in mice (compared with in vivo matured group).
“There was no difference among the groups in lifespan or in most of the physiological and behavioral analyses.”
Animalscompared with in vivo matured group
Effect of in vitro maturation of mouse oocytes on the health and lifespan of adult offspring
Human reproduction (Oxford, England)