H2B-MCHERRY

1. H2B-MCHERRY decreases H1-0 in mice (–60%, p<0.001).

   “dislodges histone H1 (–60%, p<0.001), loosening chromatin compaction”

   Animalsin adult-inducible OE miceheart

   Spatial Organizations of Heterochromatin Underlie Cardiomyocyte Nuclear Structural Integrity Against Mechanical Stress

   AHA Scientific Sessions 2025 abstract·3 Nov 2025

More on H1-0

1. H2B-MCHERRY induces cardiomyopathy in mice.

   “Constitutive H2B-mCherry OE—but not H2B or NLS-mCherry—induced progressive CM nuclear elongation and lethal cardiomyopathy.”

   Animalsin mice with constitutive CM-specific H2B-mCherry OEheart

   Spatial Organizations of Heterochromatin Underlie Cardiomyocyte Nuclear Structural Integrity Against Mechanical Stress

   AHA Scientific Sessions 2025 abstract·3 Nov 2025

More on Cardiomyopathy

1. H2B-MCHERRY activates cGAS–STING signalling in mice (↑18.3-fold, p<0.01).

   “releasing DNA that activated cGAS/STING (cGAS ↑18.3-fold, p<0.01), driving inflammation and fibrosis”

   Animalsin adult mice with inducible CM-specific H2B-mCherry OEheart

   Spatial Organizations of Heterochromatin Underlie Cardiomyocyte Nuclear Structural Integrity Against Mechanical Stress

   AHA Scientific Sessions 2025 abstract·3 Nov 2025

More on cGAS–STING signalling

1. H2B-MCHERRY decreases Young's modulus in mice (a ~50% drop in Young's modulus from 3.2→1.6kPa, p<0.001).

   “CM nuclei then softened (a ~50% drop in Young’s modulus from 3.2→1.6kPa, p<0.001)”

   Animalsin adult mice with inducible CM-specific H2B-mCherry OEheart

   Spatial Organizations of Heterochromatin Underlie Cardiomyocyte Nuclear Structural Integrity Against Mechanical Stress

   AHA Scientific Sessions 2025 abstract·3 Nov 2025

More on Young's modulus