In vivo aging-specific methylation patterns

ex vivo organoid culture is associated with in vivo aging-specific methylation patterns in mouse cells (r = 0.48, p < 0.001).

“Early-passage (P0 and P2) organoids derived from 4- and 24-month-old mice retained aging-specific methylation patterns, with a correlation coefficient of 0.48 (p < 0.001) between in vivo and ex vivo methylation differences.”

Cellsearly-passage (P0 and P2) organoidsintestinal epithelium

Modeling age-related methylation changes in vitro with intestinal organoids

BMC biology·6 Mar 2026