Isolation of human satellite cells using CD29/CDH15 and a nanoparticle-based live Pax7 mRNA detection system, and their expansion with extracellular matrix and its derivatives

Human skeletal muscle stem cells, also called satellite cells, have great potential for treating neuromuscular diseases and combating muscle aging. However, progress toward clinical use has been limited by the scarcity of donor tissue and the technical challenges of isolating, preserving, and culturing these rare cells. In this study, we introduce a new combination of cell surface markers and a gold nanoparticle-based live Pax7 mRNA detection system, both of which enable efficient isolation of human satellite cells. Additionally, we show that extracellular matrix (ECM) components support the maintenance of human satellite cells and improve their function in vivo. These findings provide integrated methods to enhance the production of human muscle-forming cells for translational and clinical applications.