Compatibility and comparative analysis of chronological and biological aging between the legacy 450K and the EPIC v2.0 arrays

Several epigenetic clocks based on DNA methylation arrays have been developed to evaluate biological aging. However, there is limited information on how the newer EPIC v2.0 arrays affect clock estimations, which lack probes initially present on legacy arrays such as the 450K array. Therefore, we analyzed how this absence affects epigenetic clock estimates using data from the InCHIANTI study. Although some clocks showed significant over- or underestimation due to probe loss, correlations between estimates from all 450K probes (unreduced) and shared probes between 450K and EPIC v2.0 (reduced) remained significant. AgeAcceleration estimates were least affected by missing probes.