Generation and characterization of two human induced pluripotent stem cell lines from young and older male donors

Advanced age is a significant risk factor for cardiovascular diseases. Previously, we reported two female human induced pluripotent stem cell (hiPSC) lines. Here, we report generation and characterization of two hiPSC lines from peripheral blood mononuclear cells (PBMCs) obtained from young (18-year-old) and older (80-year-old) male donors. The two male hiPSC lines express pluripotency markers, possess normal (46, XY) karyotypes, and have trilineage differentiation potential. Both lines genetically match their parental PBMCs. These lines provide a vital resource for regenerative medicine and development of human-specific models to understand aging-associated diseases and to investigate the epigenetic mechanisms that are involved.