Freezing
Intervention1 paper2 findings
- Animals1
Outcomes
2Membrane disruption
upin mice1Very low certainty
1 study
Membrane disruption
upin mice1Very low certainty
Freezing induces membrane disruption in mice.
Animalsunfixed tissuestraight freezing without cryoprotectantbrain
Ultrastructural comparison of fixation and cryopreservation methods for brain preservation · bioRxiv · 24 Sep 2026 · Preprint
Perivascular ice damage
upin mice1Very low certainty
1 study
Perivascular ice damage
upin mice1Very low certainty
Freezing induces perivascular ice damage in mice.
Animalsafter aldehyde fixationunprotected slow freezingbrain
Ultrastructural comparison of fixation and cryopreservation methods for brain preservation · bioRxiv · 24 Sep 2026 · Preprint
Funding
European Research Council€150k1 grant since 2026