Cell passaging

1. Cell passaging increases micronucleus in human cells.

   “The micronucleus test showed a statistically significant increase of micronucleus from the seventh passage onwards”

   Cellsfrom the seventh passage onwardsadipose tissue

   Genomic instability in long-term culture of human adipose-derived mesenchymal stromal cells

   Brazilian journal of medical and biological research = Revista brasileira de pesquisas medicas e biologicas·Jan 1, 2023

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1. Cell passaging increases DNA damage in human cells.

   “The comet assay results showed an increase in DNA damage from the fifth passage onwards.”

   Cellsfrom the fifth passage onwardsadipose tissue

   Genomic instability in long-term culture of human adipose-derived mesenchymal stromal cells

   Brazilian journal of medical and biological research = Revista brasileira de pesquisas medicas e biologicas·Jan 1, 2023

1. Cell passaging increases cellular senescence in mouse cells.

   “along with the increasing passages, the cells cultured by two methods had similar changes with a decreasing proliferation activity, increasing apoptosis rate, declining migration, diminishing stemness, and obvious senescence.”

   Cellswith increasing passagespalate

   Comparative analysis of mouse embryonic palatal mesenchymal cells isolated by two primary culture methods

   Tissue & cell·Mar 14, 2022

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1. Cell passaging decreases blastocyst formation rate in C. hircus cells.

   “the blastocyst rate was higher for cells at passages 10 and 20 than passage 30.”

   CellsEmbryos reconstructed using fibroblasts as donor cellsnuclear transfer embryoPassage 30 versus passages 10 and 20male donorss

   The expression of β-galactosidase during long-term cultured goat skin fibroblasts and the effect of donor cell passage on in vitro development of nuclear transfer embryos

   In vitro cellular & developmental biology. Animal·Mar 4, 2016

1. Cell passaging increases β-galactosidase-positive cells in C. hircus cells.

   “Irrespective of the donor age, the number of positive cells was higher in later passages from passages 20 to 50.”

   CellsIrrespective of donor ageskin fibroblastPassages 20 to 50

   The expression of β-galactosidase during long-term cultured goat skin fibroblasts and the effect of donor cell passage on in vitro development of nuclear transfer embryos

   In vitro cellular & developmental biology. Animal·Mar 4, 2016

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