CRISPR-CAS9 ribonucleoprotein

CRISPR-CAS9 ribonucleoprotein induces pcsk9 gene disruption in mice (1.39% total hepatic gene disruption).

“Systemic administration of LNPC12A9 into C57BL/6J mice led to liver tropism and a total hepatic Pcsk9 gene disruption rate of 1.39%.”

AnimalsC57BL/6J miceLNPC12A9liver

High-Throughput Screening of a Lipid Nanoparticle (LNP) Library Identifies Lead Formulations for CRISPR-Cas9 Ribonucleoprotein Delivery and Gene Editing

ACS applied materials & interfaces·16 Oct 2026

CRISPR-CAS9 ribonucleoprotein induces homology-directed repair in cells (37.8% HDR rate).

“coencapsulation of RNP and a single-stranded oligodeoxynucleotide (ssODN) template for homology-directed repair (HDR) within LNPC12A9 induced an HDR rate of 37.8%.”

CellsRNP and ssODN template coencapsulated within LNPC12A9

High-Throughput Screening of a Lipid Nanoparticle (LNP) Library Identifies Lead Formulations for CRISPR-Cas9 Ribonucleoprotein Delivery and Gene Editing

ACS applied materials & interfaces·16 Oct 2026

CRISPR-CAS9 ribonucleoprotein induces EGFP disruption in human cells (∼90% disruption at 30 nM; ∼60% at 15 nM).

“achieved ∼90% EGFP disruption in HEK293T-EGFP cells at an RNP concentration of 30 nM and ∼60% at 15 nM.”

CellsLNPC12A9HEK293T-EGFP cells30 nM and 15 nM RNP

High-Throughput Screening of a Lipid Nanoparticle (LNP) Library Identifies Lead Formulations for CRISPR-Cas9 Ribonucleoprotein Delivery and Gene Editing

ACS applied materials & interfaces·16 Oct 2026