AD-TBX18 + AD-SHOX2 + AD-TBX3

1. AD-TBX18 + AD-SHOX2 + AD-TBX3 decreases GJA1 in rat cells (log2FC=1.48).

   “while downregulating ventricular-enriched genes including Gja1 (log2FC=1.48, p <1e−50)”

   Cellscompared to VMs

   Combinatorial Transcription Factor Gene Transfer Accelerates Direct Reprogramming of Ventricular Myocytes to Pacemaker Phenotype

   AHA Scientific Sessions 2025 abstract·3 Nov 2025

More on GJA1

1. AD-TBX18 + AD-SHOX2 + AD-TBX3 increases HCN4 in rat cells (log2FC=0.27).

   “Compared to VMs, the TST group exhibited early and robust expression of SAN-specific genes such as Hcn4 (log2FC=0.27, p =1.6e−22)”

   Cellscompared to VMs

   Combinatorial Transcription Factor Gene Transfer Accelerates Direct Reprogramming of Ventricular Myocytes to Pacemaker Phenotype

   AHA Scientific Sessions 2025 abstract·3 Nov 2025

More on HCN4

1. AD-TBX18 + AD-SHOX2 + AD-TBX3 increases pacing rate in rat cells (181.6±89.5 bpm).

   “Notably, TST group showed markedly faster pacing rates as early as d3 (181.6±89.5 bpm, p<1e-50), a difference that persisted throughout d12”

   Cellsday 3n = 8

   Combinatorial Transcription Factor Gene Transfer Accelerates Direct Reprogramming of Ventricular Myocytes to Pacemaker Phenotype

   AHA Scientific Sessions 2025 abstract·3 Nov 2025