Mouse Ischemia Experiment v1

The objective of this protocol is to understand the effect of cold ischemia time on cellular senescence in mouse lung and heart tissues. The sampling of lung and heart tissues will be done based on equal weights. 6 pieces weighing around 40-50mg will be cut and put in cold preservative to conduct the time-controlled experiment. As a positive control for C12FDG a technical control of cells treated with EtOH (500µM) will be used.