Bacillus subtilis

2 papers7 findings

C, W, M, H: cells, worms or flies, mice, humans. Dark: independent labs found the same change there. Light: one lab or one study. Outline: nothing yet.

Labs are independent when their papers share no last author and no institution.

Against: findings that point the other way from most findings on the same outcome.

On aging: the drug’s trials ARN judged to be about aging, of every trial that tests it for any use.

No results posted: trials that ended more than a year ago with no results on ClinicalTrials.gov and no paper in ARN’s record.

Company ties: findings with an author at a company, or from a paper whose competing-interests statement declares ties to one.

2 independent labs in 1 country

Funders: Huazhong Agricultural University and 3 more

Animals1
Model organisms1

Outcomes

Locomotor function

upin worms2

2 studies

Bacillus subtilis improves locomotor function in worms.
“attenuated age-associated locomotor decline”
Model organisms
Multi-Model Longevity Assays Reveal Lifespan- and Healthspan-Promoting Effects of Bacillus subtilis WTC019
Microorganisms29 Jan 2026
Bacillus subtilis improves locomotor function in worms.
“aged worms fed BSTOA (BSTOA_day13) maintained better locomotor function than those fed Escherichia coli OP50 (OP50_day13).”
Model organisms
Bacillus subtilis TO-A suppresses the age-related decline in locomotion in Caenorhabditis elegans
Communications biology22 Oct 2025

Lifespan

upin mice1upin worms1+6% to +17% median lifespan

1 study

Bacillus subtilis extends lifespan in mice (+5.97% median lifespan).
“median lifespan increases of 5.97% in females and 6.05% in males”
Animalsfemales
Multi-Model Longevity Assays Reveal Lifespan- and Healthspan-Promoting Effects of Bacillus subtilis WTC019
Microorganisms29 Jan 2026
Bacillus subtilis extends lifespan in worms (+17.48% median lifespan).
“increasing median lifespan by 17.48%, 90% lifespan by 35.36%, and maximum lifespan by 19.07%”
Model organisms
Multi-Model Longevity Assays Reveal Lifespan- and Healthspan-Promoting Effects of Bacillus subtilis WTC019
Microorganisms29 Jan 2026

Senescence-associated beta-galactosidase

downin human cells1−34% decrease

1 study

Bacillus subtilis decreases senescence-associated beta-galactosidase in human cells (~34% decrease).
“reduced senescence-associated β-galactosidase activity by approximately 34%”
Cellscell lysate
Multi-Model Longevity Assays Reveal Lifespan- and Healthspan-Promoting Effects of Bacillus subtilis WTC019
Microorganisms29 Jan 2026

More on Senescence-associated beta-galactosidase

Survival

upin worms1

1 study

Bacillus subtilis increases survival in worms.
“these worms also displayed high survival rates based on the paraquat assay.”
Model organismsparaquat assay
Bacillus subtilis TO-A suppresses the age-related decline in locomotion in Caenorhabditis elegans
Communications biology22 Oct 2025

Actin structure

upin worms1

1 study

Bacillus subtilis improves actin structure in worms.
“BSTOA_day13 exhibited better activity than OP50_day13 in maintaining actin structure during age-related changes in body wall muscles.”
Model organismsduring age-related changes in body wall muscles
Bacillus subtilis TO-A suppresses the age-related decline in locomotion in Caenorhabditis elegans
Communications biology22 Oct 2025