AAK-2 and SKN-1 Are Involved in Chicoric-Acid-Induced Lifespan Extension in Caenorhabditis elegans

Chicoric acid is a dicaffeoyl ester with many bioactivities, including antioxidation, antidiabetes, and anti-inflammation. A previous study reported that chicoric acid extended the lifespan in Caenorhabditis elegans; however, the mechanism behind the effect of chicoric acid on the extended lifespan remains unknown. Consistent with the previous report, chicoric acid (25 and 50 μM) extended the maximum lifespan compared to the control (17.5 ± 3.3 and 15.6 ± 5%, respectively; p < 0.001 for both). The declines of the pumping rate and locomotive activity, two indicators of aging, were delayed by chicoric acid. Moreover, chicoric acid enhanced resistance to oxidative stress in C. elegans. It was further determined that the extended lifespan by chicoric acid was in part via aak-2 [a homologue of adenosine monophosphate (AMP)-activated protein kinase] and skn-1 (a homologue of nuclear factor erythroid 2-related factor 2). The current findings suggest that chicoric acid has the potential to be used as an anti-aging bioactive compound.