Replicative ageing

1. Replicative ageing decreases intracellular branched-chain amino acid abundance in mouse cells.

   “Branched chain amino acids L-leucine, L-isoleucine and L-valine, and L-carnitine were less abundant in aged versus control myotubes.”

   CellsP48–51 versus P7–11C2C12 myotubes

   Replicative ageing perturbs the metabolic signature of murine C 2 C 12 skeletal myotubes

   bioRxiv (Cold Spring Harbor Laboratory)·Apr 21, 2022Preprint

More on Intracellular branched-chain amino acid abundance

1. Replicative ageing increases mitochondrial superoxide production in mouse cells (Control: 2.4×10−5 ± 0.4×10−5 versus aged: 9.7×10−5 ± 1.6×10−5 RFU/sec/cell).

   “but rates of mitochondrial superoxide production were lower (Control: 2.4×10 −5 ± 0.4 × 10 −5 vs. Aged: 9.7×10 −5 ± 1.6×10 −5 RFU/sec/cell; P =0.035).”

   CellsP48–51 versus P7–11C2C12 myotubes

   Replicative ageing perturbs the metabolic signature of murine C 2 C 12 skeletal myotubes

   bioRxiv (Cold Spring Harbor Laboratory)·Apr 21, 2022Preprint

More on Mitochondrial superoxide production

1. Replicative ageing decreases mitochondrial coupling efficiency in mouse cells (Control: 79.5% versus aged: 70.3%).

   “Mitochondrial coupling efficiency (Control: 79.5 vs. Aged: 70.3%, P =0.006)”

   CellsP48–51 versus P7–11C2C12 myotubes

   Replicative ageing perturbs the metabolic signature of murine C 2 C 12 skeletal myotubes

   bioRxiv (Cold Spring Harbor Laboratory)·Apr 21, 2022Preprint