Dietary phosphorus restriction

1. Dietary phosphorus restriction improves natural breeding in rats (Colonies maintained to at least the fifth generation).

   “Continued LP feeding after weaning supported growth, gonadal development, and natural KO x KO breeding.”

   Animalsα-Klotho knockout × α-Klotho knockout breedingAt least five generations

   Low-phosphorus diet helps gene-altered rats breed for generations

   bioRxiv·Oct 18, 2026Preprint

1. Dietary phosphorus restriction improves gonadal development in rats.

   “Continued LP feeding after weaning supported growth, gonadal development, and natural KO x KO breeding.”

   Animalsα-Klotho knockoutGonad

   Low-phosphorus diet helps gene-altered rats breed for generations

   bioRxiv·Oct 18, 2026Preprint

1. Dietary phosphorus restriction improves growth in rats.

   “Continued LP feeding after weaning supported growth, gonadal development, and natural KO x KO breeding.”

   Animalsα-Klotho knockout

   Low-phosphorus diet helps gene-altered rats breed for generations

   bioRxiv·Oct 18, 2026Preprint

1. Dietary phosphorus restriction decreases plasma phosphorus in rats.

   “Maternal LP feeding also reduced plasma phosphorus in KO pups.”

   Animalsα-Klotho knockoutMaternal low-phosphorus feedingPlasmaFrom mating through lactation

   Low-phosphorus diet helps gene-altered rats breed for generations

   bioRxiv·Oct 18, 2026Preprint

More on Plasma phosphorus

1. Dietary phosphorus restriction is associated with survival to weaning in rats (92.6% versus 85.8%).

   “Maternal low-phosphorus (LP) feeding from mating through lactation was associated with higher overall survival to weaning (92.6% versus 85.8% under normal-phosphorus conditions)”

   AnimalsCompared with normal-phosphorus conditionsMaternal low-phosphorus feedingFrom mating through lactation

   Low-phosphorus diet helps gene-altered rats breed for generations

   bioRxiv·Oct 18, 2026Preprint