Nature Methods
DNA-end correction linked to better cell-free DNA profiling
The JEEPERS framework detected and corrected false methylation signals introduced when cell-free DNA was prepared for sequencing.The JEEPERS framework detected and corrected false methylation signals introduced when cell-free DNA was prepared for sequencing.
Computational studyBiomarkers
Who was studiedSimulated tumor and healthy-control cfDNA mixtures
What changedJEEPERS improved simulated cfDNA methylation reconstruction versus uncorrected data, reaching ~95% accuracy at checkpoint CpG sites
The catchJEEPERS misses jagged ends in unmethylated regions or regions lacking CpG sites