European Journal of Clinical Nutrition · Mar 29, 2022

No effect of nitrate-rich beetroot juice on microvascular function and blood pressure in younger and older individuals: a randomised, placebo-controlled double-blind pilot study

By Rogerson D, Aguilar Mora FA, Young JS et al.

BACKGROUND/OBJECTIVES: To compare the effects of supplemental inorganic nitrate (NO₃) on microvascular endothelial function and blood pressure in younger vs. older participants. SUBJECTS/METHODS: 25 individuals participated in a double-blind, randomised, placebo-controlled crossover pilot study. Participants were stratified by age (18-35 and ≥55 years) and consumed a single dose beetroot juice (providing 6.4 mmol NO₃) or NO₃-depleted beetroot juice. Blood pressure, microvascular function (via Laser Doppler Flowmetry; LDF) and urinary NO₃ were assessed, and the effects of NO₃ supplementation on cardiovascular parameters were compared between participants and conditions using mixed-design ANOVA. RESULTS: Treatments and methods were well tolerated, and no adverse events were reported. Urinary NO₃ increased 3 h following ingestion in both groups, (P = 0.02). Levels remained elevated at 24 h post consumption in younger participants only (P = 0.02). Beetroot juice had no effect on blood pressure in either group nor on microcirculatory endothelial function. CONCLUSIONS: Beetroot juice had no effect on blood pressure or microvascular endothelial function in young and older individuals. Dosage and timing regimens for supplemental beetroot juice should be avenues for further inquiry.

doi.org/10.1038/s41430-022-01115-4Open accessPubMed 35352014Interventions