Geroprotective potential of genetic and pharmacological interventions to endogenous hydrogen sulfide synthesis in Drosophila melanogaster

Endogenous hydrogen sulfide (H₂S) is a gasotransmitter with a wide range of physiological functions. Aging is accompanied by disruption of H₂S homeostasis, therefore, interventions to the processes of H₂S metabolism to maintain its balance may have geroprotective potential. Here we demonstrated the additive geroprotective effect of combined genetic and pharmacological interventions to the hydrogen sulfide biosynthesis system by overexpression of cystathionine-β-synthase and cystathionine-γ-lyase genes and treatment with precursors of H₂S synthesis cysteine (Cys) and N-acetyl-L-cysteine (NAC). The obtained results suggest that additive effects of genetic and pharmacological interventions to H₂S metabolism may be associated with the complex interaction between beneficial action of H₂S production and prevention of adverse effects of excess H₂S production by Cys and NAC treatment.