International Journal of Molecular Sciences · Sep 10, 2025

Correlation Between the Proportion of Senescence-Associated β-Galactosidase-Stained CD8+ T Cells and Age: A Cross-Sectional Study in Japan

By Tsubokawa M, Shimizu Y, Yazaki M et al.

Recently, senescent T cells in the peripheral blood have been detected using senescence-associated β-galactosidase (SA-βGal) activity and have been used as an endpoint in clinical trials. However, the epidemiological association between the abundance of SA-βGal-stained senescent CD8+ T cells and chronological age has not been fully elucidated. To examine the correlation between the proportion of SA-βGalhigh CD8+ T cells and age, we analyzed previously collected clinical trial data. We conducted a cross-sectional analysis of 632 Japanese adults aged 40-59 years who participated in the screening phase of a clinical trial. To characterize senescent CD8+ T cells, we measured the proportion of SA-βGalhigh in total CD8+ T cells and each subset-naïve, central memory (TCM), effector memory (TEM), and terminally differentiated effector memory (TEMRA). We then calculated the correlation coefficients between the proportion of SA-βGalhigh CD8+ T cells and age. The proportion of SA-βGalhigh cells in total CD8+ T cells, naïve, TCM, TEM, and TEMRA CD8+ T cells increased significantly with age. In Japanese adults, the proportion of SA-βGalhigh in CD8+ T cells may serve as a useful biomarker of immune senescence.

doi.org/10.3390/ijms26188799Open accessPubMed 41009368Biomarkers