The Science of the Total Environment · Dec 16, 2021

Intermittent multi-generational effects of 1-hexyl-3-methylimidazolium nitrate on Caenorhabditis elegans mediated by lipid metabolism

By Zhang J, Feng L

Ionic liquids (ILs) become environmental pollutants. Their environmental toxicities included inhibitory effects, stimulatory ones and hormesis combining both aspects on various organisms. However, the mechanisms still need systematic investigations. Presently, 1-hexyl-3-methylimidazolium nitrate ([Hmim]NO₃) was chosen as one representative IL. Its effects on lifespan and reproduction were studied on C. elegans with lipid metabolism as the potential mechanism. Two types (A and B) intermittent multi-generational exposure arrangements were set up to mimic realistic ILs exposure scenarios. Type A arrangement had exposure in F1, F5 and F9 with recovery in T2-T4, T6-T8 and T10-T12, and type B arrangement had exposure in F1, F3, F5, F7, F9 and F11 with recovery in T2, T4, T6, T8, T10 and T12. In type A exposure, the effects of [Hmim]NO₃ on reproduction were 1.32-, 1.68-, 1.23-, 0.96-, 1.68-, 1.07-, 1.25-, 1.64-, 1.31-, 1.11-, 0.89- and 1.02-fold of the control in F1, T2-T4, F5, T6-T8, F9, T10-T12, respectively. The results showed oscillation between stimulatory and inhibitory effects. In type B exposure, the effects showed fewer inhibitions and more stimulations across generations. Moreover, the effects on reproduction showed trade-off relationships with lifespan, and the trade-off was more obvious in type B exposure. Further biochemical and gene expression analysis showed that lipid metabolism was closely related with lifespan and reproduction in type A exposure, but it was connected with cholesterol synthesis in type B exposure. The results demonstrated different strategies in the biological responses to the two arrangements of intermittent multi-generational exposure.

doi.org/10.1016/j.scitotenv.2021.152479PubMed 34923010Mechanisms