Nan Fang Yi Ke Da Xue Xue Bao · Jun 30, 2019

Correlation between methylation level of CDKN2A and CDKN2B genes and aging in healthy individuals

By Zheng Z, Ji H, Chen C et al.

OBJECTIVE: To analyze the relationship between CDKN2A and CDKN2B gene methylation with aging in the general population. METHODS: We collected peripheral blood samples from 284 male and 246 female healthy subjects for detection of methylation levels of CDKN2A and CDKN2B genes using quantitative methylation-specific PCR (qMSP). The relationship between the methylation levels of CDKN2A and CDKN2B genes and aging was analyzed using Spearman or Pearson correlation test. RESULTS: We found a significant positive correlation between the methylation levels of the two genes in these subjects (P < 0.05). In the overall population as well in the female subjects, CDKN2A methylation was found to be inversely correlated with age (P < 0.05). The methylation levels of CDKN2A and CDKN2B genes were inversely correlated with TG, ApoE, Lp(a) and AST in the overall population (P < 0.05). In both the female and male subjects, the methylation levels of the two genes were inversely correlated with Lp(a) (P < 0.05). In the male subjects, CDKN2A methylation was inversely correlated with AST (P < 0.05), while CDKN2B methylation was inversely correlated with HDL and ApoE (P < 0.05). In the female subjects, CDKN2A methylation was positively correlated with LDL and inversely correlated with ApoE and AST (P < 0.05). CONCLUSIONS: The methylation levels of CDKN2A and CDKN2B are closely related to age and the levels of multiple proteins in healthy subjects.

doi.org/10.12122/j.issn.1673-4254.2019.06.15PubMed 31270053Biomarkers