Methods in Molecular Biology · 2019

Assessment of Young and Aged Hematopoietic Stem Cell Activity by Competitive Serial Transplantation Assays

By Zhang YW, Cabezas-Wallscheid N

Healthy hematopoietic stem cells (HSCs) are capable to self-renew and reconstitute the complete hematopoietic system. Upon aging, there is an increased incidence of blood-related diseases. Age-related phenotypes have been widely studied by bone marrow transplantation experiments, where reconstitution of the transplanted cells is a direct measure of HSC activity. In this protocol we describe a competitive bone marrow transplantation assay to functionally test young and old HSCs.

doi.org/10.1007/978-1-4939-9574-5_15PubMed 31197778Mechanisms