Preprint · Oct 22, 2024

Yale University adapted Akoya Phenocycler-Fusion Tissue Staining and Imaging Protocol for Fresh Frozen Human Lymph node Samples adapted from Indiana University v1

By Farzad N, Enninful A, Fan R

This protocol presents the Yale University TMC Akoya Phenocycler-Fusion Tissue Staining & Imaging Protocol for Fixated Fresh Frozen Human Lymph Node Samples. This protocol was adapted from Indiana University adapted Akoya Phenocycler-Fusion Tissue Staining and Imaging Protocol for Fresh Frozen Kidney Samples. This protocol has been used for FF human lymph node samples for the Cellular Senescence Network (SenNet) Program and the Human BioMolecular Atlas Program consortia. The size of the marker panels has ranged between 35 to 47 targets and includes cell type markers for B cells, T cells, macrophages, and other immune cell types. that will label various cell types Multiple sections have been placed on a single slide and imaged simultaneously.

doi.org/10.17504/protocols.io.ewov19erylr2/v1Open accessAI & data