Protocol collection for Saurat et al. CSC 2024 "Genome-wide CRISPR screen identifies neddylation as a regulator of neuronal aging and AD neurodegeneration" v2

This is a protocol collection for the publication "Genome-wide CRISPR screen identifies neddylation as a regulator of neuronal aging and AD neurodegeneration" Saurat N et al. Cell Stem Cell 2024 The collection consists of 19 different protocols: hPSC culture using E8 and EDTA-based passaging Engineering of H9iCAS-APPswe/swe cell lines Generation of H9 Nurr1-GFP LRRK2 G2019S knock-in line Cortical neuron differentiation Whole genome CRISPR Cas9 screen in PSC-derived neurons Data analysis for Pooled CRISPR screen RNA extraction and qPCR Immunocytochemistry High content imaging Aβ ELISA Western blotting Fractionation of protein lysates into sarkosyl soluble/insoluble fractions Secondary Validation of CRISPR-screen targets Generation of UBA3 and NAE1 overexpression lentiviruses Flow Cytometry Aβ42 neurotoxicity assays Proteosome activity assay Presto Blue Viability Assay CCK8 Viability Assay Full manuscript can be found here: DOI:10.1016/j.stem.2024.06.001