Preprint · Aug 7, 2022

URMC TriState SenNet Mouse Lung Digestion v2

By Kaur G, Rahman I

The objective of this document is to share the material and kits used and steps involved in digesting the mouse lungs to perform scRNA analyses. This study was performed for the TriState SenNet TMC Bioanalyses Core at the University of Rochester, as part of the Cellular Senescence Network Program (SenNet). This protocol ensures minimum damage to the cells in suspension and ensures maximum viability to conduct downstream analyses.

doi.org/10.17504/protocols.io.5qpvok9z9l4o/v2Open accessAI & data