bioRxiv · Nov 5, 2021
Age-induced P-bodies become detrimental and shorten the lifespan of yeast
By Choi J, Wang S, Li Y et al.
Aging is an irreversible process characterized by a progressive loss of homeostasis in cells, which often manifests as protein aggregates. Recently, it has been speculated that aggregates of RNA-binding proteins (RBPs) may go through pathological transitions during aging and drive the progression of ageassociated neurodegenerative diseases. Using Saccharomyces cerevisiae as a model system of aging, we find that P-bodies —an RBP granule that is formed and can be beneficial for cell growth during stress conditions — naturally form during aging without any external stresses and an increase in P-body intensity is negatively correlated with the future lifespan of yeast cells. When mother cells transfer age-induced P-bodies to daughter cells, the mother cells extend lifespan, while the daughter cells grow poorly, suggesting that these age-induced P-bodies may be directly pathological. Furthermore, we find that suppressing acidification of the cytosol during aging slows down the increase in the intensity of P-body foci and extends lifespan. Our data suggest that acidification of the cytosol may facilitate the pathological transition of RBP granules during aging.